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We further conducted stratified analysis to estimate the level of knowledge of radiation in different characteristics people around the plant by investigating respondents whether understood the conception of radiation. The options of answer were divided into five levels: No idea, Do not quite understand, Understand some, Understand more and Very familiar, which corresponding scored 1, 2, 3, 4, 5 point. Respondents were required to selecting one answer out of five options and the higher mean score indicating the higher level of understand. As shown in Table 2, men's mean score was slightly higher than women's (p<0.001); the town residents' mean score was also higher than rural residents' (p<0.001). In addition, for different age groups, marital status, educational level, family income and distance away from the plant, the mean score was also different, respectively. We further used the correlation analysis to understand the relationship between different groups and different scores. In our results, we can find that there is a negative correlation between age and the level of understanding (r=-0.12, p<0.001), but with the improvement in educational level and family income, the level of knowledge of radiation is corresponding increase (r=0.43, p<0.001 and r=0.18, p<0.001, respectively). However, we failed to find any significant correlation between different marital status or distance away from the plant and the level of knowledge of radiation (see Table 2).. tissue expression pattern of a single transcript. Since the 1980s, in. Results obtained in preclinical and clinical studies strongly suggest that consumption of irradiated food may induce chromosomal abnormalities and lethal mutations in rapidly dividing cells (39-48). An addition of irradiated sucrose to the growth medium resulted with genome instability in human lymphocytes (39). Chromosomes of these lymphocytes were grossly damaged (appeared broken or fragmented) and their mitosis were suppressed (39). Similarly, fragmentation of chromosomes accompanied with inhibited mitosis were noticed in human leucocytes when standard growth medium was replaced by irradiated one (40).. For Western blotting, the cultured cardiomyocytes were homogenised in lysis buffer (150 mM NaCl, 1.0% nonyl phenoxypolyethoxylethanol-40, and 50 mM Tris HCl; ElpisBio, Korea) containing protease inhibitor (Sigma-Aldrich, USA) and clarified by centrifugation at 13,000 rpm for 15 minutes at 4°C to collect the supernatant. The proteins in the supernatant were resolved by 7.5% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad) and separated. They were transferred to a polyvinylidene difluoride membrane (PVDF; Millipore, USA) using transfer apparatus for 2 hours at 300 mA. The membrane was blocked with 5% BSA for 2 hours at room temperature and incubated with primary antibodies against mouse-RACK1 (Santa Cruz Biotechnology, USA at 4°C overnight. The membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Abcam, USA). The protein was detected using an LAS-3000 imaging system (Fujifilm, Japan). For re-probing, the blots were stripped with western Blot Stripping Buffer (Thermo Scientific, USA). The blot was then re-blocked and re-probed with rabbit-cleaved caspase-3 (Santa Cruz Biotechnology, USA), mouse-Bcl-2 (Santa Cruz Biotechnology, USA) and rabbit-glyceraldehyde 3-phosphate dehydrogenase (GAPDH, Sigma-Aldrich, USA) antibody detected with ECL substrate and LAS-3000 imaging system (Fujifilm, Japan)..

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The SwRI Human Head Surrogate (HHS) is developed and applied for assessing behind helmet blunt trauma injuries. This human head surrogate is designed to fill the void between Post-Mortem Human Subject testing which has biofidelity but handling restrictions and commercial ballistic head forms which have little biofidelity but are easy to use. This unique human head surrogate is based on refreshed human craniums and surrogate materials representing human head soft tissues such as the skin, dura, and brain. A methodology for refreshing the craniums is developed and verified through material testing. An experimental methodology based on the HHS is developed and used in a series of experiments in which non-perforating ballistic impact of combat helmets is performed. Sensors embedded in the human head surrogates allow for direct measurement of intracranial pressure, cranial strain, and head/helmet acceleration. Experiments include both baseline combat helmets and helmets with a supplemental ceramic applique for addressing larger caliber threats. Based on a relatively large number of tests, the SwRI Human Head Surrogate has demonstrated great potential for providing insights in to injury mechanics resulting from non-perforating ballistic impact of combat helmets, allowing for a direct measure and assessment of behind helmet blunt trauma injuries.. Neuropathic pain (NP) has been recently redefined by the Neuropathic Pain Special Interest Group (NeuPSIG) as pain arising as a direct consequence of a lesion or disease affecting the somatosensory system [1]. Although there have been progresses in several studies on its mechanism and treatment buy Seroquel no prescription low cost NP remains a type of pain which is clinically refractory. Pulsed radiofrequency (PRF) is a minimally invasive technique and differs from continuous radiofrequency (CRF) in several aspects. The radiofrequency current emitted in PRF has an interim period of 480 msec following each 20 msec emission, which allows heat to disperse to the surrounding tissues so that the temperature of the therapy target site will not exceed 42 ℃ through which it could avoid a series of side effects caused by irreversible nerve damage in the case of CRF [2]. Since its invention, PRF has been proven by an array of clinical studies in treating kinds of NP, including postherpetic neuralgia [3], painful diabetic neuropathy [4], trigeminal neuralgia [5, 6], etc.. genes in combination with the regulation of their activity mechanisms. as increased levels of adrenalin..
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