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PCR-RFLP was used to determine the A2589G and G3456C DNA polymorphisms of the ABCA1 gene. The study group was analyzed for potential clinical predictors of low HDL-C.. born ..
in a way that shows this.. A total of 1902 patients who received HD for at least six months (ranging from seven months to twenty six years, with a median of 6.7 years) were recruited from fifteen institutes, including one medical center, three regional hospitals, and eleven dialysis clinics in southern Taiwan. Patients who rejected giving the informed consent and those who were in acute illness were excluded, and finally 1681 prevalent patients were included for this study. All patients received 3.5-4.5 hours per session, three sessions per week maintenance HD. Of the 1559 patients with known primary renal diseases, the causes of renal failure were as followed: chronic glomerulonephritis (n=644, 41.3%), diabetic nephropathy (n=579, 37.1%), hypertensive renal disease (n=88, 5.6%), lupus nephropathy (n=28, 1.8%), gouty nephropathy (n=25, 1.6%), obstructive nephropathy (n=23, 1.5%), polycystic kidney disease (n=28, 1.8%) and others (n=144, 8.6%). All the eligible subjects were tested for both anti-HCV and HBsAg examinations. The processing of all the samples in our study followed a regulated rule, and analyzed in a same laboratory by the same technician specialized in viral hepatitis nucleic acid measurement. Pre-HD blood samples were collected monthly in the period between January, 2011 and January, 2012. Only patients testing positive for HBsAg or anti-HCV at least twice within 6 months were eligible for the study. HBsAg was determined by using a standard chemiluminescent microparticle immunoassay (ARCHITECT HBsAg, Abbott Diagnostics). Anti-HCV was examined using a third-generation enzyme immunoassay (Abbott Laboratories, North Chicago, IL). HBV DNA was determined by a standardized automated quantitative polymerase chain reaction assay (COBAS TaqMan HBV test; Roche Diagnostics; detection limit 12 IU/ml) (13). HCV RNA was measured by a real-time polymerase chain reaction assay (RealTime HCV; Abbott Molecular, Des Plaines IL, USA; detection limit: 12 IU/ml) (14). All the above-mentioned tests were duplicated for each tested sample..
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Diabetic nephropathy is a leading cause of chronic kidney disease (CKD). In diabetes, changes in serum levels of both soluble alpha Klotho (sKL) and fibroblast growth factor 23 (FGF-23) have been associated with CKD progression..
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